Back

ⓘ Hanes–Woolf plot. In biochemistry, a Hanes–Woolf plot is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration ..




Hanes–Woolf plot
                                     

ⓘ Hanes–Woolf plot

In biochemistry, a Hanes–Woolf plot is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration. It is based on the rearrangement of the Michaelis–Menten equation shown below:

+{K_{m} \over V_{\max }}}

As is clear from the equation, perfect data will yield a straight line of slope 1/ V max, a y -intercept of K m / V max and an x -intercept of − K m.

Like other techniques that linearize the Michaelis–Menten equation, the Hanes–Woolf plot was used historically for rapid determination of the important kinetic parameters K m, V max and V max / K m, but it has been superseded by nonlinear regression methods that are significantly more accurate and no longer computationally inaccessible. It remains useful, however, as a means to present data graphically.

One drawback of the Hanes–Woolf approach is that neither ordinate nor abscissa represent independent variables: both are dependent on substrate concentration. As a result, the typical measure of goodness of fit, the correlation coefficient R, is not applicable.

                                     
  • timeless and eternal. Another important Taliesin text is the Ystoria Taliesin, Hanes Taliesin, which contains Welsh prose and poetry, about and, claiming to

Users also searched:

...
...
...